The patient-professional relationship is a major administrative, medical, and social factor that deserves attention by both patients and healthcare professionals and could influence health outcomes. An important factor in the successful implementation of World Health Organization and Palestinian guidelines for management of type II diabetes is the patient-professional relationship. The objectives of this study were to assess the patient-professional relationship and its relationship with patient characteristics and organizational factors in patients with type II diabetes. A cross-sectional study was conducted, adopting the Patient Perception of Diabetes Care Quality Questionnaire to assess organizational factors, and using the Patient Satisfaction with Professional Scale (PSPS) that included physicians, nurses, and dieticians to assess patient-professional relationship. Descriptive and comparative statistics were used to describe patient characteristics. All analyses were performed using SPSS v 16.0. Three hundred thirty patients with type II diabetes were enrolled in the study. The mean age of participants was 60 ± 9.7 years; 51.2% were male. The mean PSPS score was lower than the average score (mean = 38.5 ± 15.9; cumulative percentage = 48.1%). There was a significant difference in the median PSPS scores among patients received instructions from healthcare professionals (Kruskal-Wallis test; p <0.05). The Spearman's rank order correlation coefficients showed a significant correlation of patient-professional relationship with preventive monitoring, preventive education, facilitating the patient-professional relationship, and collaboration. Instruction from healthcare professionals (p <0.001), preventive education (p <0.001), facilitating patient-professional relationship (p = 0.001), and collaboration (p <0.05) were still statistically significantly related to patient-professional relationship after adjusting for covariates using multiple linear regression. As a conclusion, patient-professional relationship was inappropriate. Instruction and guidance may be an important barrier for achieving appropriate patient-professional relationship. Further improvement of guidance and educational programs that emphasize the role of healthcare providers could be helpful in a patient-professional relationship