Aims: The aim of this study was to investigate the clinical profile of ICU patients with Acinetobacter baumannii infection and to assess susceptibility profile of A. baumannii to various tested antibiotics. Method: Retrospective analysis of electronic data of patients admitted to An-Najah National University hospital during the study period from 2015 to 2017. All information regarding culture and sensitivity tests were extracted from the electronic data of the ICU unit. Results: In total, 99 cases of positive A. baumannii cultures were identified during the study period. The mean ± SD age of the identified cases was 59.9 ± 18.8 (range: 15 – 87 years). The majority of cases were males (58; 58.6%). Out of 99 cases, 46 (46.5%) died. Approximately 75% of the cases had the infection during their stay in the hospital (nosocomial infections). Twenty-one different anti-microbial agents were tested against A. baumannii. Colistin was the most effective with 98.9% sensitivity. Tigecyline also showed high efficacy (75.9%) against A. baumannii but lesser than that recorded for Colistin. Other less effective antimicrobial agents included rifampin, minocycline, and co-trimoxazole. There was no significant association between sensitivity profile for the anti-microbial agents and the following variables: gender, age, health status, and number of comorbid diseases. However, there was significant association between sensitivity profile and time factor as well as source of infection. Conclusion: Strict infection control policy and rational use of antibiotics are needed to control A. baumannii infections and reduce development of future resistance in A. baumannii.