Introduction

Throughout history, therapeutically active plant products have received substantial attention due to their valuable role in the discoveries of specific medications. The aim of this study was to assess, for the first time, the antimicrobial, antioxidant, antilipase, anti-α-amylase and cytotoxic properties of four fractions derived from Anchusa ovata Lehm. (AO) leaves.

Methods

Antioxidant, antilipase and anti-amylase potentials of (AO) were established using DPPH (1,1-diphenyl-2-picrylhydrazyl), p-nitrophenyl butyrate and dinitro-salicylic acid procedures, respectively, while antimicrobial activity was conducted using broth microdilution assay against eight Gram-positive, Gram-negative bacterial strains in addition to one fungal strain. Moreover, the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] cytotoxic assay was utilized against cervical cancer cells (HeLa).

Results

The methanol fraction of AO showed potential antioxidant, antilipase, and α-amylase inhibitory activities with IC₅₀ values of 9.55 ± 0.13, 53.7 ± 0.41 and 16.55 ± 1.84 μg/ml, respectively compared with the positive controls Trolox, Orlistat and Acarbose that had IC₅₀ values of 3.23 ± 0.92, 12.3 ± 0.35 and 28.18 ± 1.22 μg/ml, respectively. Moreover, the hexane, acetone, and methanol fractions had wide ranges of antimicrobial potential. In addition, the cytotoxic activity outcomes which showed the best activity was for the aqueous followed by acetone, hexane and methanol fractions with IC₅₀ values of 1.04, 2.72, 3.96 and 17.67 mg/ml, respectively.

Conclusion

Our data demonstrate a wide range of biological characteristics for each AO plant fraction. This profiling information about the methanol fraction provided important data for further research and pharmaceutical applications.

Title

European Journal of Integrative Medicine

Publisher

Elsevier GmbH

Publisher Country

United Kingdom

Indexing

Scopus

Impact Factor

1.0

Publication Type

Both (Printed and Online)

Volume

34

Year

12

Pages

101066