Antibacterial, Anticandidal, Phytochemical, and Biological Evaluations of Pellitory Plant
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Original research

Pellitory plant (Parietaria judaica(PJ)) is one of the most widely used Arabian traditional medicinal plants due to its ability to cureseveral infectious diseases and other illnesses. The current study is aimed at assessing the phytoconstituents, antilipase, antiamylase,antimicrobial, and cytotoxic characters of the Pellitory plant (Parietaria judaica(PJ)). Phytochemical screening and procyanidindetection were conducted according to the standard phytochemical procedures. Porcine pancreatic lipase andα-amylaseinhibitory activities were carried out usingp-nitrophenyl butyrate and dinitrosalicylic acid assays, respectively. In addition,antimicrobial activity was determined utilizing a microdilution assay against several bacterial and fungal strains. Besides, thecytotoxic effect against HeLa cell line was tested employing 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. The quantitative test results revealed that the methanol fraction of PJ contains18:55±0:55mg of procyanidin and has a potentialα-amylase inhibitory activity compared with the antidiabetic drug Acarbose withIC50values of15:84±2:25and28:18±1:22μg/ml, respectively. Also, it has a potential antilipase activity compared to thecommercial antiobesity drug, Orlistat, with IC50values of38:9±0:29and12:3±0:35μg/ml, respectively. The acetone, hexane,and methanol fractions have broad-spectrum antibacterial activity against the screened bacterial strains, while the acetonefraction has shown anticandidal activity with a MIC value of 0.195 mg/ml. The PJ hexane and acetone fractions decreased HeLacell viability significantly (pvalue<0.0001) by approximately 90% at the concentration of 0.625 mg/ml. The revealed outcomesshowed that the methanol fraction has strongα-amylase and lipase inhibitory characters. Besides, acetone, hexane, andmethanol fractions have broad-spectrum antibacterial activity, while the acetone fraction revealed potent antifungal activityagainstCandida albicans. Moreover, at low concentrations, hexane and acetone fractions have potent cytotoxic andantiproliferative activity against HeLa cancer cells. Nevertheless, PJ acetone, hexane, and methanol fractions can serve as aneffective source of natural products to develop new antiobesity, antidiabetic, antimicrobial, and anticancer agents.
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BioMed Research International
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