Phytochemical profile and in vitro antioxidant, antimicrobial, vital physiological enzymes inhibitory and cytotoxic effects of Artemisia jordanica leaves essential oil from Palestine
Publication Type
Conference Paper
Authors

Artemisia jordanica (AJ) is one of the folkloric medicinal plants and grows in the arid condition used by Palestinian Bedouins in the Al-Naqab desert for the treatment of diabetes and gastrointestinal infections. The current investigation aimed, for the first time, to characterize and quantify the (AJ) essential oil (EO) phytoconstituents and evaluate EO’s antioxidant, anti-obesity, antidiabetic, antimicrobial, anti-inflammatory, and cytotoxic activities. The gas chromatography-mass spectrometer (GC-MS) technique was utilized to characterize the chemical ingredients of (AJ) EO, while 1,1diphenyl2picrylhydrazyl0(DPPH) procedure was used to assess the antioxidant potential. Moreover, validated biochemical approaches were utilized to evaluate the inhibitory action against core metabolic enzymes implicated in obesity (pancreatic lipase) and diabetes (α-amylase and α-glucosidase). Furthermore, the microbicidal efficacy of (AJ) EO was measured against seven microbial strains utilizing the broth microdilution assay. Besides, the cytotoxic activity of (AJ) EO was estimated utilizing the (MTS) procedure against human breast0(MCF-7), cervical (HeLa), liver (Hep3B), and colon (Caco-2) tumor cell lines. Finally, the anti-inflammatory activity of (AJ) EO was measured utilizing a COX inhibitory screening test kit on human recombinant cyclooxygenase-2 (COX-2) and bovine cyclooxygenase-1. The analytical investigation revealed the presence of 12 molecules. Oxygenated terpenoids, including bornyl acetate (69.28%) and endo-borneol (19.39%) presented as major components of the (AJ) EO. The EO exhibited potent antioxidant activity compared with Trolox, with IC50 (half-maximal inhibitory concentration) doses of 2.18±0.24 and 1.58±1.49 µg/mL, respectively, while it showed a weak anti-lipase effect compared with orlistat, with IC50 doses of 51.41±0.91 and 0.13±0.86 μg/mL, respectively. In addition, the tested EO displayed a potent α-amylase suppressing effect compared with the positive control, acarbose (IC50=14.17±0.39 and 8.53± 0.72 μg/mL, respectively). Notably, the (AJ) EO exhibited strong α-glucosidase inhibitory potential compared with the positive control, acarbose0(IC50=144.45±0.88 and 62.36±1.05 μg/mL, respectively). The results of the MTS assay revealed that the EO had has a cytotoxic effect against all the screened tumor cells. Notably, (AJ) EO showed potent antimicrobial properties, with higher antibacterial potentials against Methicillin-Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, and Proteus vulgaris, with a minimum inhibitory concentration (MIC) of 0.625 μg/mL than the commercial antibiotic ciprofloxacin which has a MIC of 12.5, 0.78, and 15 μg/mL, respectively. Moreover, it showed a ten-fold more potent antifungal activity against Candida albicans than the commercial anticandidal drug fluconazole. Besides, the EO inhibited the enzymes COX-1 and COX-2 (IC50 of 15.64 and 91.91 µg/mL, respectively), compared with the anti-inflammatory drug ketoprofen (IC50 of 7.89 and 40.18 µg/mL, respectively). The (AJ) EO has strong antioxidant, antibacterial, antifungal, anti-α-amylase, anti-α-glucosidase, and COX inhibitory effects which could be a favorite candidate for the treatment of various neurodegenerative diseases caused by harmful free radicals, microbial resistance, diabetes, and inflammations. Further in-depth investigations are urgently crucial to explore the importance of such medicinal plants in pharmaceutical production.

 

Conference
Conference Title
3rd International Congress on Plant Biology
Conference Country
Turkey
Conference Date
Sept. 21, 2022 - Sept. 23, 2022
Conference Sponsor
http://iconpb2022.erdogan.edu.tr/Files/ckFiles/iconpb2022-erdogan-edu-tr/IConPB%20Kongre%20Kitab%C4%B1%2007122022.pdf
Additional Info
Conference Website