Asymmetry diamine ligand was used to selectively prepare the trans-stereoisomer dimer of type [Br(N⋂N’)Cu(µBr)2Cu(N⋂N’)Br] in a one-pot and high-yield sonosynthesis procedure. The trans-[Br(N⋂N’)Cu(µBr)2Cu(N⋂N’)Br] square pyramidal structure was proved by XRD-crystal structure. Moreover, several physicochemical tools including EDX, FAB-MS, UV-vis, FT-IR and CHN-EA were used to check the dimer formula. Both Cu(II) ions exhibited one Br, 2half-bonds via 2µ-Br and two N-diamine to complete the five-coordinated bonds and form trans-Br2-trans-(N'N’) complex with square pyramidal geometry. Additionally, the classical N-H…Br and non-classical CMe-H…Br H-bonds interactions formed the novel S(9) synthons stabilizing the lattice of the dimer as inferred from the TG/DTG result. Molecular Electrostatic Potential (MEP), and HSA/XRD-interactions analysis compatible with chemical content and supported the formation of such synthons in the trans-stereoisomer-dimer lattice. The complex's solvatochromism and halochromism showed their sensitivity and proto-responsibility to the dissolved medium, respectively. Experimentally, the dimer been LOX enzyme inhibition more effectively than COX one, such seen was explained theoretically by molecular docking.