To develop amathematical model describing the behavior of chromophore complexes, the main factors influencing the stability of poloxamine and poloxamer complexes were determined. Gel permeation chromatography, ultraviolet absorbance measurements and FT-IR were used for the analysis. It was found that the studied complexes in chlorinated organic solvents are unstable in the presence of oxygen, air, or wa-ter. The developed mathematical model allows for more effective planning and execution of experiments due to the possibility of predicting UV absorption based on the distribution of complexes in chlorinated solvents.Keywords: poloxamines, poloxamers, block copolymers, ammonium ferrothiocyanate, colorimetric analysis, mathematical modeling.