Background: Ecballium elaterium is a Mediterranean plant that grows wild in Palestine and is re ported to have a wide range of biological activities. Methods: In the ongoing study, the total phenol content using Folin–Ciocalteu assay, antioxidant activity using DPPH assay and anticancer activity using MTT and LDH assays against skin mela noma (B16–F1) cell line of the E. elaterium leaf aqueous extract were evaluated. Results: The estimated phenol content by Folin-Ciocalteu method was equal to 48.5 mg/g in GA equivalent. Moreover, the recorded DPPH scavenging power of E. elaterium aqueous extract (27.8 ± 0.13 %) with 66.2 ± 0.41 µg/mL IC50 value. Additionally, the cytotoxic and cytostatic anticancer activity of this extract against skin melanoma was determined using MTT and LDH assays. Re garding the obtained MTT results, the aqoueus extract from E. elaterium was not significantly cytotoxic or cytostatic effective compared to the control, but still a significant difference between the extract cytostatic and cytotoxic effect. Furthermore, LDH data showed that this extract signifi cantly worked as a cytotoxic and cytostatic agent with no significant difference between its cytotox ic and cytostatic activity. Conclusion: E. elaterium leaf aqueous extract was found to be a possible effective resource for anti cancer and antioxidant medications.

Title

Jordan Journal of Applied Science Natural Science Series

Publisher

Applied Science Private University

Publisher Country

Jordan

Publication Type

Both (Printed and Online)

Volume

18

Year

2024

Pages

48-52