Objective: The current study aimed to identify the nature of the relationship between the Big Five personality factors and self-awareness among students at An-Najah National University. It also aimed to identify the level of each of the Big Five personality factors and self-awareness, and the impact of each of the independent variables on the study variables. Methodology: The study included (250) students (74 males and 176 females) distributed across different colleges. Data were collected using the Self-Awareness Scale and the Big Five Personality Traits Scale. Results: The study found that four personality traits were widely spread among students, with neuroticism appearing at a low level. Conclusion: University students have a high degree of self-awareness, according to the results. The study also found a positive relationship between conscientiousness and self-awareness, as well as a negative relationship between neuroticism and self-awareness. Females were more neurotic than males.