Background: This study conceptualizes supply chain ambidexterity through two capabilities, supply chain adaptability and agility. Accordingly, it investigates the impact of supply chain adaptability and agility on green product innovation (GPI) and supply chain sustainability in Jordanian manufacturing firms. It also examines the mediating role of GPI in these relationships. The study is based on dynamic capabilities theory (DCT) as the theoretical foundation. Methods: A quantitative research approach was employed, with data collected from 346 supply chain managers using a structured questionnaire. Partial Least Squares Structural Equation Modeling (PLS-SEM) was used for analysis. Results: The findings reveal that supply chain adaptability does not directly influence sustainability but significantly enhances GPI, which positively impacts sustainability. Supply chain agility, however, directly and significantly improves both GPI and sustainability, highlighting its importance in achieving sustainable supply chain performance. Additionally, GPI mediates the relationship between supply chain ambidexterity and sustainability, reinforcing its role as a key enabler of eco-friendly supply chain management. These findings provide theoretical and managerial implications. Conclusions: The study extends DCT by confirming the role of GPI in linking supply chain ambidexterity to sustainability. Managers should prioritize agility, invest in sustainable products, and adopt green practices to enhance competitiveness.