Inhibitory Effect of Meropenem Antibiotic on the Activity of Glutathione-S-Transferases
Publication Type
Original research
Authors

The following experiment investigated the effect of meropenem antibiotic on the activity of glutathione-S-transferase (GST) isoenzymes. This effect was estimated spectrophotometrically at different concentrations of meropenem using 1-chloro-2,4-dinitrobenzene (CDNB) as a substrate. The obtained results revealed that meropenem antibiotic demonstrated an inhibitory effect on GST activity at low tested concentrations (0.25, 0.5, 1 and 2.5 mM) that resulted in 30.5, 39.4, 45.1, and 100% inhibition, respectively. Also, results provided that the meropenem concentration required to reduce the GST activity to half equal to 1.266 mM. After applying the Line weaver-Burk blot and equation, it was noted that both Km and Vmax values decreased in the presence of meropenem, indicating uncompetitive inhibition. In conclusion, meropenem antibiotic is a potential inhibitor for GST. Therefore, it will be crucial to consider the metabolic defense systems when selecting the dosages of this antibiotic to be utilized for the treatment of infections.

Journal
Title
Palestinian Medical and Pharmaceutical Journal
Publisher
An-Najah National University
Publisher Country
Palestine
Indexing
Scopus
Impact Factor
None
Publication Type
Both (Printed and Online)
Volume
10
Year
2025
Pages
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