To develop an adventitious regeneration system for pear cultivars. several experiments were con¬ducted with 2 cultivars of Pyrus com-tunls L. ('Seckel' and 'Louise Bonne") and one cultlvar of P^ brotschnelderl Bend. ('Crystal Pear'). Half-leaves, taken from shoots proliferating on Lepolvre medium, were plated in petri-dishes on medium supplemented with various combinations of cytoklnlns and auxins. Cultures of the above cultivars had been established Tron nature trees. Among the growth regulators tested, thidlaxuron (TDZ), combined with naphthalene-acetic acid (NAA), was the most efficient for stimu¬lation of adventitious shoots. The optimum level of TDZ was about 3 uH; shoot regeneration was observed over a wide range of TDZ and HAA concentrations (0.5 to 5 uM and 2.5 to 13 ua. respectively). Among dif¬ferent aacronutricnt compositions, y/2 and 1/* Mura-shlgc and Skoog were the most effective. Sucrose concentrations (to to 50 g L-1) had a linear signifi¬cant effect on shoot regeneration or 'Crystal Pear*.

Title

Plant Cell Reports (1989) 7:688-691

Publisher

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Publisher Country

Palestine

Publication Type

Both (Printed and Online)

Volume

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Year

1989

Pages

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