This study examined the effect of reused treated grey water on yield of 10 pearl millet accessions (Pennisetum glaucum) in An-Najah University, Tulkarm, Palestine. Ten pearl millet seeds were planted during May 2010 in each separate plastic containers (35 x 50 x 15 cm) filled with 45 kg sandy clay soil in four replication of a completely randomized design and irrigated with three types of water (Fresh water as control, raw grey water, treated grey water). Results clearly show that there were no significant differences in the yield under different water treatments. Also no significant interaction was observed between water treatments and cultivars. However, accessions IP3616, IP22269, Sudan pop II, and Sudan pop I gave high total fresh weight fresh weight of straw while accessions IP3616 and IP22269 produced the highest fresh weight of straw. The accessions IP6104, IP6110, IP19612 and ICMV155 registered the highest plant height.