Borago officinalis. plant is an important plant of high medicinal and nutritional values. This study designed to evaluate antioxidant activity, screen the existence of phytogenic chemical compounds and to determine the total flavonoid and phenol contents of wild and cultivated Borago officinalis. Total flavonoid contents of the wild and cultivated Borago officinalis were determined by using rutin reference standard method and total phenols determined by using Folin Ciocalteu’s method while antioxidant activity evaluated by using 2, 2-diphenyl-1-picryl-hydrazyl-hydrate assay. Phytochemical analyses indicated the presence of carbohydrate, phenols, flavonoids, phytosteroids tannins and volatile oil. The total flavonoid content of the methanolic extract from the wild Borage plant was 22.4mg RU/g this value was reduced to 13.1mg RU/g for the cultivated methanolic extract as well as the total phenols contents was dropped from 5.21mg GA/g to 2.37mg GA/g methanolic extracts. Total tannins content of the wild growing Borage plant methanolic extract was 13.7mg CA/g this value was increased in the cultivated Borage to 21.33mg CA/g. The wild leaves extract had IC50 = 6.3μg/ml for wild leaves extract was closer to IC50 value of Trolox (standard reference with high antioxidant activity), while the cultivated leaves extract had higher IC50= 8.7μg/ml which mean lower antioxidant activity than the wild growing one. The data of this study showed that the extracts of Borago officinalis possess antioxidant and free radical scavenging activities. Variation was clear between wild and cultivated plants, These exhibited properties propose that such plant extract can could be used as natural preservatives in both food and pharmaceutical industries. Therefore, more research are necessary to investigate different cultural practices on the efficiency of borage plant.